Norovirus (GⅠ) RT-PCR Setsi sa Tlhahlobo

Tlhaloso e Khutšoanyane:

E loketse ho fumanoa ha Norovirus (GⅠ) ho shellfish, meroho e tala le litholoana, metsi, mantle, mahlatsa le mehlala e meng.Ho ntšoa ha asiti ea nucleic ho lokela ho etsoa ka lisebelisoa tsa nucleic acid kapa mokhoa o tobileng oa pyrolysis ho latela mefuta e fapaneng ea mohlala.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la Sehlahiswa

Norovirus (GⅠ) RT-PCR Detection Kit (Lyophilized)

Boholo

48test/kit, 50tests/kit

Tšebeliso e reriloeng

E loketse ho fumanoa ha Norovirus (GⅠ) ho shellfish, meroho e tala le litholoana, metsi, mantle, mahlatsa le mehlala e meng.Nucleic acid extraction e lokela ho etsoa ka nucleic acid extraction kit kapa mokhoa o tobileng oa pyrolysis ho latela mefuta e fapaneng ea mohlala. The kit ke ALL-READY PCR SYSTEM(Lyophilized), e nang le enzyme ea amplification ea DNA, reverse transcriptase,reaction buffer, primers tse khethehileng. le lisebelisoa tse hlokahalang bakeng sa ho lemoha PCR ea fluorescent.

Litaba tsa Sehlahisoa

Likaroloana Sephutheloana tlhaloso Motsoako
Norovirus (GⅠ) PCR Mix 1 × botlolo (phofo ea Lyophilized)  50Tlhahlobo dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 hantle-strip tube(Lyophilized) 48Tlhahlobo
Taolo e Ntle 1 * 0.2ml tube (lyophilized)  10Liteko

Plasmide e nang le likaroloana tse khethehileng tsa Norovirus (GⅠ).

Tharollo e qhalang 1.5 di ml Cryotube 500uL /
Taolo e Fosahetseng 1.5 di ml Cryotube 200uL 0.9%NaCl

Storage & Shelf Life

(1) Thepa e ka tsamaisoa ka mocheso oa kamore.

(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.

(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.

(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.

Lisebelisoa

GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.

Setšoantšo sa ts'ebetso

a) Mofuta oa botlolo:

1

b) Mofuta oa 8 oa hantle-strip tube:

2

Pcr Amplification

E khothalelitsoeTlhophiso

Mohato Baesekele Mocheso (℃) Nako Mocha oa fluorescence
1 1 50 8 mets  
2 1 95 2 mets  
3 40 95 5s  
60 10s Bokella fluorescence ea FAM

*Tlhokomeliso: Matshwao a dikanale tsa fluorescence tsa FAM a tla bokellwa ho 60 ℃.

Ho toloka Liphetho tsa Teko

Channel

Tlhaloso ea liphetho

Mokhatlo oa FAM

Ct≤35

Norovirus (GⅠ) e ntle

Undet

Norovirus (GⅠ) Negative

35

Resuite e belaetsang, leka hape*

*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.


  • E fetileng:
  • E 'ngoe:

  • Lihlahisoa tse Amanang