E.coli O157:H7 PCR sete ya ho lemoha

Tlhaloso e Khutšoanyane:

Escherichia coli O157:H7 (E.coli O157:H7) ke baktheria ea gram-negative ea leloko la Enterobacteriaceae, e hlahisang bongata bo bongata ba chefo ea Vero.


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la Sehlahiswa

E.coli O157:H7 PCR sethitho sa ho lemoha (Lyophilized)

Boholo

48test/kit, 50tests/kit

Tšebeliso e reriloeng

Escherichia coli O157:H7 (E.coli O157:H7) ke baktheria ea gram-negative ea leloko la Enterobacteriaceae, e hlahisang bongata bo bongata ba chefo ea Vero.Ho ea ka meriana, hangata ho hlaha ka tšohanyetso ka mahlaba a bohloko ka mpeng le letšollo le nang le metsi, ho lateloa ke letšollo la mali matsatsing a seng makae hamorao, le ka lebisang feberung kapa ho se be le feberu, le lefu maemong a matla.Setsi sena se loketse tlhahlobo ea boleng ba Escherichia coli O157: H7 lijong, lisampole tsa metsi, mantle, mahlatsa, mokelikeli o matlafatsang baktheria le lisampole tse ling ho sebelisoa molao-motheo oa PCR ea nako ea nnete. Lyophilized), e nang le enzyme ea amplification ea DNA, buffer ea karabelo, li-primers le li-probes tse hlokahalang bakeng sa ho lemoha PCR ea fluorescent.

Litaba tsa Sehlahisoa

Likaroloana Sephutheloana tlhaloso Motsoako
E.coli O157:H7 PCR Mix 1 × botlolo (phofo ea Lyophilized)  50Tlhahlobo dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 hantle-strip tube(Lyophilized) 48Tlhahlobo
Taolo e Ntle 1 * 0.2ml tube (lyophilized)  10Liteko

Plasmid e nang le E.coli O157: H7 likaroloana tse khethehileng

Tharollo e qhalang 1.5 di ml Cryotube 500uL /
Taolo e Fosahetseng 1.5 di ml Cryotube 200uL 0.9%NaCl

Storage & Shelf Life

(1) Thepa e ka tsamaisoa ka mocheso oa kamore.

(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.

(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.

(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.

Lisebelisoa

GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.

Setšoantšo sa ts'ebetso

a) Mofuta oa botlolo:

1

b) Mofuta oa 8 oa hantle-strip tube:

2

Pcr Amplification

E khothalelitsoeTlhophiso

Mohato Baesekele Mocheso (℃) Nako Mocha oa fluorescence
1 1 95 2 mets  
2 40 95 5s  
60 10s Bokella fluorescence ea FAM

*Tlhokomeliso: Letšoao la kanale ea fluorescence ea FAM le tla bokelloa ho 60 ℃.

Ho toloka Liphetho tsa Teko

Channel

Tlhaloso ea liphetho

Mokhatlo oa FAM

Ct≤35

E.coli O157:H7 E ntle

Undet

E.coli O157:H7 Negative

35

Resuite e belaetsang, leka hape*

*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.


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