中文Listeria monocytogenes PCR Detection Kit
Lebitso la Sehlahiswa
Listeria monocytogenes PCR Detection Kit
Boholo
48test/kit, 50tests/kit
Tšebeliso e reriloeng
Listeria monocytogenes ke microbacterium ya gram-positive e ka holang pakeng tsa 4℃ le 45℃.Ke e 'ngoe ea likokoana-hloko tse ka sehloohong tse sokelang bophelo ba batho lijong tse nang le sehatsetsing.Lipontšo tse ka sehloohong tsa tšoaetso ke septicemia, meningitis le mononucleosis.Setsi sena se loketse ho lemoha boleng ba Listeria monocytogenes lijong, lisampole tsa metsi, mantleng, mahlatsa, mokelikeli o matlafatsang baktheria le lisampole tse ling ho sebelisoa molao-motheo oa nako ea nnete ea fluorescence PCR. The kit is an ALL-READY PCR SYSTEM(Lyophilized) , e nang le enzyme ea amplification ea DNA, reverse transcriptase, reaction buffer, li-primers tse khethehileng le li-probes tse hlokahalang bakeng sa ho lemoha PCR ea fluorescent.
Storage & Shelf Life
(1) Thepa e ka tsamaisoa ka mocheso oa kamore.
(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.
(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.
(4) The lyophilized powder version reagent e lokela ho bolokoa ka -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.
Litaba tsa Sehlahisoa
| Likaroloana | Sephutheloana | tlhaloso | Motsoako |
| Listeria monocytogenes PCR Mix | 1 × botlolo (phofo ea Lyophilized) | 50Tlhahlobo | dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 hantle-strip tube(Lyophilized) | 48Tlhahlobo | ||
| Taolo e Ntle | 1 * 0.2ml tube (lyophilized) | 10Liteko | Plasmide e nang le likhechana tse khethehileng tsa Listeria monocytogenes |
| Tharollo e qhalang | 1.5 di ml Cryotube | 500uL | / |
| Taolo e Fosahetseng | 1.5 di ml Cryotube | 200uL | 0.9%NaCl |
Lisebelisoa
GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.
Setšoantšo sa ts'ebetso
a)
b)
Pcr Amplification
E khothalelitsoeTlhophiso
| Mohato | Baesekele | Mocheso (℃) | Nako | Mocha oa fluorescence |
| 1 | 1 | 50 | 8 mets | |
| 2 | 1 | 95 | 2 mets | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Bokella fluorescence ea FAM |
*Tlhokomeliso: Matshwao a dikanale tsa fluorescence tsa FAM a tla bokellwa ho 60 ℃.
Ho toloka Liphetho tsa Teko
| Channel | Tlhaloso ea liphetho |
| Mokhatlo oa FAM | |
| Ct≤35 | Listeria monocytogenes e ntle |
| Undet | Listeria monocytogenes Negative |
| 35 | Resuite e belaetsang, leka hape* |
*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.
