中文E.coli O157:H7 PCR sete ya ho lemoha
Lebitso la Sehlahiswa
E.coli O157:H7 PCR sethitho sa ho lemoha (Lyophilized)
Boholo
48test/kit, 50tests/kit
Tšebeliso e reriloeng
Escherichia coli O157:H7 (E.coli O157:H7) ke baktheria ea gram-negative ea leloko la Enterobacteriaceae, e hlahisang bongata bo bongata ba chefo ea Vero.Ho ea ka meriana, hangata ho hlaha ka tšohanyetso ka mahlaba a bohloko ka mpeng le letšollo le nang le metsi, ho lateloa ke letšollo la mali matsatsing a seng makae hamorao, le ka lebisang feberung kapa ho se be le feberu, le lefu maemong a matla.Setsi sena se loketse tlhahlobo ea boleng ba Escherichia coli O157: H7 lijong, lisampole tsa metsi, mantle, mahlatsa, mokelikeli o matlafatsang baktheria le lisampole tse ling ho sebelisoa molao-motheo oa PCR ea nako ea nnete. Lyophilized), e nang le enzyme ea amplification ea DNA, buffer ea karabelo, li-primers le li-probes tse hlokahalang bakeng sa ho lemoha PCR ea fluorescent.
Litaba tsa Sehlahisoa
| Likaroloana | Sephutheloana | tlhaloso | Motsoako |
| E.coli O157:H7 PCR Mix | 1 × botlolo (phofo ea Lyophilized) | 50Tlhahlobo | dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 hantle-strip tube(Lyophilized) | 48Tlhahlobo | ||
| Taolo e Ntle | 1 * 0.2ml tube (lyophilized) | 10Liteko | Plasmid e nang le E.coli O157: H7 likaroloana tse khethehileng |
| Tharollo e qhalang | 1.5 di ml Cryotube | 500uL | / |
| Taolo e Fosahetseng | 1.5 di ml Cryotube | 200uL | 0.9%NaCl |
Storage & Shelf Life
(1) Thepa e ka tsamaisoa ka mocheso oa kamore.
(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.
(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.
(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.
Lisebelisoa
GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.
Setšoantšo sa ts'ebetso
a) Mofuta oa botlolo:
b) Mofuta oa 8 oa hantle-strip tube:
Pcr Amplification
E khothalelitsoeTlhophiso
| Mohato | Baesekele | Mocheso (℃) | Nako | Mocha oa fluorescence |
| 1 | 1 | 95 | 2 mets | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Bokella fluorescence ea FAM |
*Tlhokomeliso: Letšoao la kanale ea fluorescence ea FAM le tla bokelloa ho 60 ℃.
Ho toloka Liphetho tsa Teko
| Channel | Tlhaloso ea liphetho |
| Mokhatlo oa FAM | |
| Ct≤35 | E.coli O157:H7 E ntle |
| Undet | E.coli O157:H7 Negative |
| 35 | Resuite e belaetsang, leka hape* |
*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.
