RT-PCR Detection Kit

Tlhaloso e Khutšoanyane:

Setsi sena se sebelisa mokhoa oa 'nete oa nako ea fluorescent RT-PCR ho lemoha RNA ea Porcine reproductive and breathing virus virus nucleic acid discovery kit(PRRSV) linthong tsa lefu la lisele tse kang lithane, li-lymph nodes le spleen le lisebelisoa tsa lefu la metsi tse kang ente le mali. ea likolobe .


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Lebitso la Sehlahiswa

RT-PCR kit (Lyophilized) +

Boholo

48test/kit, 50tests/kit

Tšebeliso e reriloeng

Setsi sena se sebelisa mokhoa oa 'nete oa nako ea fluorescent RT-PCR ho lemoha RNA ea Porcine reproductive and breathing virus virus nucleic acid discovery kit(PRRSV) linthong tsa lefu la lisele tse kang lithane, li-lymph nodes le spleen le lisebelisoa tsa lefu la metsi tse kang ente le mali. ea likolobe .E loketse ho fumanwa, ho hlahlojwa le ho fuputsa epidemiological ya Porcine Blue Ear Virus.Thepa ke ALL-READY PCR SYSTEM(Lyophilized), e nang le reverse transcriptase, DNA amplification enzyme, reaction buffer, primers le probes tse hlokahalang bakeng sa ho lemoha RT-PCR ea fluorescent.

Litaba tsa Sehlahisoa

Likaroloana Sephutheloana tlhaloso Motsoako
Motsoako oa PBEV PCR 1 × botlolo (phofo ea Lyophilized)  50Tlhahlobo dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 hantle-strip tube(Lyophilized) 48Tlhahlobo
Taolo e Ntle 1 * 0.2ml tube (lyophilized)  10Liteko

Plasmid kapa Pseudovirus e nang le likaroloana tse khethehileng tsa PRRSV

Tharollo e qhalang 1.5 di ml Cryotube 500uL /
Taolo e Fosahetseng 1.5 di ml Cryotube 200uL 0.9%NaCl

Storage & Shelf Life

(1) Thepa e ka tsamaisoa ka mocheso oa kamore.

(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.

(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.

(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.

Lisebelisoa

GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.

Setšoantšo sa ts'ebetso

a) Mofuta oa botlolo:

1

b) Mofuta oa 8 oa hantle-strip tube:

2

Pcr Amplification

Recommended Setting

Mohato

Baesekele

Mocheso (℃)

Nako

Mocha oa fluorescence

1

1

48

8 mets

/

2

1

95

2 mets

/

3

40

95

5s

/

60

10s

Bokella fluorescence ea FAM

*Tlhokomeliso: Matshwao a dikanale tsa fluorescence tsa FAM a tla bokellwa ho 60 ℃.

Ho toloka Liphetho tsa Teko

Channel

Tlhaloso ea liphetho

Mokhatlo oa FAM

Ct≤35

Vibrio Parahaemolyticus Positive

Undet

Vibrio Parahaemolyticus Negative

35

Resuite e belaetsang, leka hape*

*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.


  • E fetileng:
  • E 'ngoe:

  • Lihlahisoa tse Amanang