中文Seteishene sa ho lemoha seoa sa letšollo la kokoana-hloko ea RT-PCR
Lebitso la Sehlahiswa
RT-PCR Detection Kit (Lyophilized) ea kokoana-hloko ea seoa sa letšollo
Boholo
48test/kit, 50tests/kit
Tšebeliso e reriloeng
Setsi sena se sebelisa mokhoa oa sebele oa nako ea fluorescent RT-PCR ho lemoha RNA ea kokoana-hloko ea letšollo ea Porcine epidemic (PEDV) linthong tsa lefu la lisele tse kang tonsils, lymph nodes le spleen le lisebelisoa tsa lefu la metsi tse kang ente le mali a likolobe.E loketse ho lemoha, ho hlahloba le ho etsa lipatlisiso tsa epidemiological virus ea letšollo la seoa sa Porcine.Kit ke ALL-READY PCR SYSTEM(Lyophilized), e nang le reverse transcriptase, DNA amplification enzyme, reaction buffer, primers le probes tse hlokahalang bakeng sa ho lemoha RT-PCR ea fluorescent.
Litaba tsa Sehlahisoa
| Likaroloana | Sephutheloana | tlhaloso | Motsoako |
| PEDV PCR Mix | 1 × botlolo (phofo ea Lyophilized) | 50Tlhahlobo | dNTPs, MgCl2, Primers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 hantle-strip tube(Lyophilized) | 48Tlhahlobo | ||
| Taolo e Ntle | 1 * 0.2ml tube (lyophilized) | 10Liteko | Plasmid kapa Pseudovirus e nang le likaroloana tse khethehileng tsa PEDV |
| Tharollo e qhalang | 1.5 di ml Cryotube | 500uL | / |
| Taolo e Fosahetseng | 1.5 di ml Cryotube | 200uL | 0.9%NaCl |
Storage & Shelf Life
(1) Thepa e ka tsamaisoa ka mocheso oa kamore.
(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.
(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.
(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.
Lisebelisoa
GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.
Setšoantšo sa ts'ebetso
a) Mofuta oa botlolo:
b) Mofuta oa 8 oa hantle-strip tube:
Pcr Amplification
Recommended Setting
| Mohato | Baesekele | Mocheso (℃) | Nako | Mocha oa fluorescence |
| 1 | 1 | 48 | 8 mets | / |
| 2 | 1 | 95 | 2 mets | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Bokella fluorescence ea FAM |
*Tlhokomeliso: Matshwao a dikanale tsa fluorescence tsa FAM a tla bokellwa ho 60 ℃.
Ho toloka Liphetho tsa Teko
| Channel | Tlhaloso ea liphetho |
| Mokhatlo oa FAM | |
| Ct≤35 | PEDV e ntle |
| Undet | PEDV Negative |
| 35 | Sephetho se belaetsang, leka hape* |
*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.
