中文Kokoana-hloko ea lefu la leoto le molomo RT-PCR Setsi sa ho Khetholla
Lebitso la Sehlahiswa
Kokoana-hloko ea lefu la leoto le molomo RT-PCR Detection Kit (Lyophilized)
Boholo
48test/kit, 50tests/kit
Tšebeliso e reriloeng
Setsi sena se sebelisa mokhoa oa nako ea sebele oa fluorescent RT-PCR ho lemoha RNA ea lefu la leoto le molomo(CSFV) linthong tsa lefu la lisele tse kang tonsils, lymph nodes le spleen le lisebelisoa tsa lefu la metsi tse kang ente le mali a likolobe.E loketse ho lemoha, ho hlahloba le ho etsa lipatlisiso tsa epidemiological ea lefu la maoto le molomo.Thepa ke ALL-READY PCR SYSTEM(Lyophilized), e nang le enzyme ea amplification ea DNA, reverse transcriptase, reaction buffer, li-primers tse khethehileng le li-probes tse hlokahalang bakeng sa ho lemoha PCR ea fluorescent.
Litaba tsa Sehlahisoa
| Likaroloana | Sephutheloana | tlhaloso | Motsoako |
| Motsoako oa FMD PCR | 1 × botlolo (phofo ea Lyophilized) | 50Tlhahlobo | dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 hantle-strip tube(Lyophilized) | 48Tlhahlobo | ||
| Taolo e Ntle | 1 * 0.2ml tube (lyophilized) | 10Liteko | Plasmid e nang le likaroloana tse khethehileng tsa FMD |
| Tharollo e qhalang | 1.5 di ml Cryotube | 500uL | / |
| Taolo e Fosahetseng | 1.5 di ml Cryotube | 200uL | 0.9%NaCl |
Storage & Shelf Life
(1) Thepa e ka tsamaisoa ka mocheso oa kamore.
(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.
(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.
(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.
Lisebelisoa
GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.
Setšoantšo sa ts'ebetso
a) Mofuta oa botlolo:
b) Mofuta oa 8 oa hantle-strip tube:
Pcr Amplification
E khothalelitsoeTlhophiso
| Mohato | Baesekele | Mocheso (℃) | Nako | Mocha oa fluorescence |
| 1 | 1 | 50 | 8 mets | |
| 2 | 1 | 95 | 2 mets | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Bokella fluorescence ea FAM |
*Tlhokomeliso: Matshwao a dikanale tsa fluorescence tsa FAM a tla bokellwa ho 60 ℃.
Ho toloka Liphetho tsa Teko
| Channel | Tlhaloso ea liphetho |
| Mokhatlo oa FAM | |
| Ct≤35 | FMD e ntle |
| Undet | FMD Negative |
| 35 | Sephetho se belaetsang, leka hape* |
*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.
