中文Afrika swine fever virus PCR kit ea ho lemoha
Lebitso la Sehlahiswa
Afrika swine fever virus kit ea ho lemoha PCR (Lyophilized)
Boholo
48test/kit, 50tests/kit
Tšebeliso e reriloeng
Sesebelisoa sena se sebelisa mokhoa oa PCR oa nako ea 'nete oa fluorescent ho lemoha DNA eaAfrika swine fever virus (ASFV)ka thepa ea lefu la lisele tse kang tonsils, lymph nodes le spleen le lisebelisoa tsa lefu la metsi tse kang ente le mali a likolobe.E loketse bakeng sa ho lemoha, ho hlahloba le ho etsa lipatlisiso tsa epidemiological eaAfrika swine fever virus.Kit ke ALL-READY PCR SYSTEM(Lyophilized), e nang le enzyme ea amplification ea DNA, buffer ea reaction, li-primers tse khethehileng le li-probes tse hlokahalang bakeng sa ho lemoha PCR ea fluorescent.
Litaba tsa Sehlahisoa
| Likaroloana | Sephutheloana | tlhaloso | Motsoako |
| Motsoako oa ASFV PCR | 1 × botlolo (phofo ea Lyophilized) | 50Tlhahlobo | dNTPs, MgCl2, Primers, Lipatlisiso, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 hantle-strip tube(Lyophilized) | 48Tlhahlobo | ||
| Taolo e Ntle | 1 * 0.2ml tube (lyophilized) | 10Liteko | Plasmid e nang le likaroloana tse khethehileng tsa ASFV |
| Tharollo e qhalang | 1.5 di ml Cryotube | 500uL | / |
| Taolo e Fosahetseng | 1.5 di ml Cryotube | 200uL | 0.9%NaCl |
Storage & Shelf Life
(1) Thepa e ka tsamaisoa ka mocheso oa kamore.
(2) Bophelo ba sethala ke likhoeli tse 18 ho -20 ℃ le likhoeli tse 12 ho 2 ℃ ~ 30 ℃.
(3)Sheba leibole ho kit bakeng sa letsatsi la tlhahiso le letsatsi la ho felloa ke nako.
(4) The lyophilized powder version reagent e lokela ho bolokoa ho -20 ℃ ka mor'a ho qhibiliha le ho qhoqhoa ho pheta-pheta - ho qhibiliha ho lokela ho ba ka tlase ho makhetlo a 4.
Lisebelisoa
GENECHECKER UF-150, UF-300 ea nako ea sebele ea fluorescence PCR sesebelisoa.
Setšoantšo sa ts'ebetso
a) Mofuta oa botlolo:
b) Mofuta oa 8 oa hantle-strip tube:
Pcr Amplification
E khothalelitsoeTlhophiso
| Mohato | Baesekele | Mocheso (℃) | Nako | Mocha oa fluorescence |
| 1 | 1 | 95 | 2 mets | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Bokella fluorescence ea FAM |
*Tlhokomeliso: Matshwao a dikanale tsa fluorescence tsa FAM a tla bokellwa ho 60 ℃.
Ho toloka Liphetho tsa Teko
| Channel | Tlhaloso ea liphetho |
| Mokhatlo oa FAM | |
| Ct≤35 | ASFV e ntle |
| Undet | ASFV Negative |
| 35 | Sephetho se belaetsang, leka hape* |
*Haeba sephetho sa tlhahlobo ea mocha oa FAM se na le boleng ba Ct ≤40 'me se bontša mokhoa o tloaelehileng oa ho holisa sebopeho sa "S", sephetho se hlalosoa e le se setle, ho seng joalo se fosahetse.
